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human whole genome oligo microarray service  (Arraystar inc)

 
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    Arraystar inc human whole genome oligo microarray service
    Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to <t>microarray</t> gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.
    Human Whole Genome Oligo Microarray Service, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human whole genome oligo microarray service/product/Arraystar inc
    Average 90 stars, based on 1 article reviews
    human whole genome oligo microarray service - by Bioz Stars, 2026-05
    90/100 stars

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    1) Product Images from "MiR-4674 regulates angiogenesis in tissue injury by targeting p38K signaling in endothelial cells"

    Article Title: MiR-4674 regulates angiogenesis in tissue injury by targeting p38K signaling in endothelial cells

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00542.2019

    Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to microarray gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.
    Figure Legend Snippet: Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to microarray gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.

    Techniques Used: Transfection, Negative Control, Microarray, Real-time Polymerase Chain Reaction, Western Blot, Immunoprecipitation, Quantitative RT-PCR, Expressing, Luciferase, Activity Assay



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    human whole genome oligo microarray service  (Arraystar inc)
    90
    Arraystar inc human whole genome oligo microarray service
    Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to <t>microarray</t> gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.
    Human Whole Genome Oligo Microarray Service, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human whole genome oligo microarray service/product/Arraystar inc
    Average 90 stars, based on 1 article reviews
    human whole genome oligo microarray service - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

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    Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to microarray gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: MiR-4674 regulates angiogenesis in tissue injury by targeting p38K signaling in endothelial cells

    doi: 10.1152/ajpcell.00542.2019

    Figure Lengend Snippet: Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to microarray gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.

    Article Snippet: For DNA microarray gene chip analysis, HUVECs were transfected with 30 nM miRNA negative control or miR-4674 mimics for 24 h. Cells were collected into RNeasy mini kit (Qiagen) and sent for two-color, 4 × 44 K format (Agilent Technologies) human whole genome oligo microarray service (ArrayStar).

    Techniques: Transfection, Negative Control, Microarray, Real-time Polymerase Chain Reaction, Western Blot, Immunoprecipitation, Quantitative RT-PCR, Expressing, Luciferase, Activity Assay